Binh Thanh Thai
Doctor of Philosophy, Research Candidate
Arafura Timor Research Facility
Charles Darwin University, Darwin, North Territory
Tel: +61 (0) 88920 9293;
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Project title: Molecular Genetic studies of Common carp (Cyprinus carpio L.)
Supervisor: Prof.Christopher M Austin
Common carp (Cyprinus carpio L.) is one of the most important cultured fish species, with world annual production over three million metric tons. The natural distribution of the species is generally thought to encompass Western Europe through Eurasia to China, Korea, Japan and South East Asia including Vietnam, and from Siberia to the Mediterranean and India. However, due to its popularity as an aquaculture and ornamental species, common carp has also been widely translocated, both within and outside its nature distribution. Domesticated strains and feral populations are now established in many countries including Australia and America.
Summary of project
The exploitation, management and conservation of carp genetic resources are complicated by the long history of domestication and the frequent often global translocation of carp stocks. There is also considerable debate concerning the origin of the major form of carp, their taxonomy and their contribution to aquaculture, especially in Asia. There have been number of genetic studies on common carp using a range of approaches, including both traditional selective breeding and molecular genetic procedures. The comparative value of these studies is however limited by the diversity of genetic approaches used, their usually narrow geographic focus, and often limited sampling.
As with many other countries, common carp is one of the most important culture species in Vietnam. It is the oldest cultivated fish species in this country, with the first records of domestication dating back at least a thousand years. Traditional selective breeding of common carp in Vietnam has a long history with a certain degree of success. However, there has been no application of modern molecular techniques to the quantification of genetic variation in Vietnamese common carp stocks. There are three major components to this study. First, genetic diversity of common carp stocks will be examined on a global scale using a phylogenetic analysis of mitochondrial nucleotide sequences. By using samples representing major strains obtained throughout the species geographical range a global genealogy of carp will be generated. A phylogenetic representation of nucleotide variation amongst populations of common carp will identify genetically distinct forms of potential value to aquaculture, and help establish relationships among strains and their possible origin. Secondly, this study will quantify genetic variation in Vietnamese carp populations in detail using samples collected from both the wild and hatcheries, to investigate the degree of strain mixing and identify possible inbred populations.
Lastly, genetic relationship between common carp and other major fish species in cyprinid family in Vietnam were examined by using sequence of mitochondrial DNA Cytochrome b, D-loop, and 16S rRNA gene regions.